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Vivalavida

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    Immunology

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Lepton

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  1. Hiii again, Can someone tell me how can I transfer some NKT lymphocyte from a mouse to another without irradiating the donor mouse... I mean, will there be any reject because of mismatching between the donor and the recipient ? How can I prevent this ??? Tks for any help ....
  2. Hiii everyone, I really need some help... I would like to see if my γδ T-cells are activated or not, I've heard about the γδ T-cell isolation kit from Miltenyi BUT the problem is that they use an anti-γδ TCR antibody for the purification, and I'm afraid that this antibody will activate the cells while fixing thus make my results be wrong ... What am I supposed to do ?? Do I have to analyse by excluding all the other lymphocytes and then use an activating marker or what ,? Reallly need some help Tks
  3. Hiii everyone, I really need some help... I would like to see if my γδ T-cells are activated or not, I've heard about the γδ T-cell isolation kit from Miltenyi BUT the problem is that they use an anti-γδ TCR antibody for the purification, and I'm afraid that this antibody will activate the cells while fixing thus make my results be wrong ... What am I supposed to do ?? Do I have to analyse by excluding all the other lymphocytes and then use an activating marker or what ,? Reallly need some help Tks
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