i am doing research on hypertension, and mainly test on the function of aortic rings of hypertensive rats. Sometimes, i can not get a full contraction (>0.5g tension) induced by phenylephrine(PE,10-7M) but i can get the >0.5g tension contraction induced by high k(KCl,80mM) in the same ring. i have not incubate any drugs yet. Please tell me what's wrong with the rings? Thanks.

i am doing research on hypertension, and mainly test on the function of aortic rings of hypertensive rats. Sometimes, i can not get a full contraction (>0.5g tension) induced by phenylephrine(PE,10-7M) but i can get the >0.5g tension contraction induced by high k(KCl,80mM) in the same ring. i have not incubate any drugs yet. Please tell me what's wrong with the rings? Thanks.

review your methodology again. it isn't the rings

Thanks for your kindly reply.

 

If any mistake in methodology, then all rings (4 rings in one set) should have the same result, but it's only 1-2 rings gave me the result as i mentioned previous.And sometimes, i got the vice versus result also....>0.5g of PE and <0.5g of high K. However, i would like to ask that is it the interval timing ( for adding the drugs like PE and high K) very important?

 

Thank you.

 

 

 

 

review your methodology again. it isn't the rings