Kevsen Posted February 28, 2011 Share Posted February 28, 2011 (edited) I want to genotype E18 mice as fast as possible. Preferably within a couple of hours. I suppose the bottleneck of this all will be the tissue digestion/ DNA extraction. Does anyone have experience with this and/ or can provide with some digestion tips? What tissue you use? what buffer you use? Fastest I did it until now was 2 hrs , shaking @55 celcius with: 10% Triton 10x MGB H2O Proteinase K Thans in advance guys! Edited February 28, 2011 by Kevsen Link to comment Share on other sites More sharing options...
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