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Kevsen

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Lepton

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  1. I want to genotype E18 mice as fast as possible. Preferably within a couple of hours. I suppose the bottleneck of this all will be the tissue digestion/ DNA extraction. Does anyone have experience with this and/ or can provide with some digestion tips? What tissue you use? what buffer you use? Fastest I did it until now was 2 hrs , shaking @55 celcius with: 10% Triton 10x MGB H2O Proteinase K Thans in advance guys!
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