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High Speed genotyping/ DNA extraction?

Kevsen
in Genetics

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I want to genotype E18 mice as fast as possible. Preferably within a couple of hours.

 

I suppose the bottleneck of this all will be the tissue digestion/ DNA extraction.

 

Does anyone have experience with this and/ or can provide with some digestion tips? What tissue you use? what buffer you use?

 

Fastest I did it until now was 2 hrs , shaking @55 celcius with:

 

10% Triton

10x MGB

H2O

Proteinase K

 

 

Thans in advance guys!

Edited by Kevsen

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