Jump to content

Spectrophotometry

qzno
in Biochemistry and Molecular Biology

Featured Replies

I have a quick Spectrophotometry question!

 

A solution of the nucleotide base uracil, in a 1mm cuvette, has an absorbance at 260 nm of 0.65. Pure solvent in a matched quartz cuvette has an absorbance of 0.07. What is the molar concentration of the uracil solution? Assume the molar absorption coefficient is 8.2 x 103 M-1 cm-1

Well, this looks suspiciously like homework.

All you need to do is plug the figures into your basic equation, so what part are you stuck on precisely?

 

If it's not homework: was the spectrophotometer "zeroed" using clear solvent before measuring the uracil solution?

Edited by Greippi

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.