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qzno

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September 21, 2010
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September 22, 2010

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Spectrophotometry

qzno posted a topic in Biochemistry and Molecular Biology

I have a quick Spectrophotometry question! A solution of the nucleotide base uracil, in a 1mm cuvette, has an absorbance at 260 nm of 0.65. Pure solvent in a matched quartz cuvette has an absorbance of 0.07. What is the molar concentration of the uracil solution? Assume the molar absorption coefficient is 8.2 x 103 M-1 cm-1
- September 21, 2010
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