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what is the correction factor 260 for TAMRA and ROX

shu zhou
in Biochemistry and Molecular Biology

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I purchased fluorescent labeled RNA. And I want to detect the RNA concentration using 260nm reading. But I need subtract the fluorophore effect at 260 nm using equation A260corre=A260-(Amax*C.F.260), where i can find the C.F.260 for different fluorophores? Especially TAMRA and ROX?

 

Thanks a lot

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