Shadowness Posted October 28, 2009 Share Posted October 28, 2009 Simple question, the gas chromatography graph shows two curves to show the ratio of two products. Will the retention time for each product always be in the same order for the two products? I am asking because we pooled class data, and although the ratios are the relatively the same, the order of retention time was different. For instance: Data 1: 1.04min 40% 1.06min 60% Data 2: 1.04min 60% 1.06min 40% Can anyone explain? Thanks. Link to comment Share on other sites More sharing options...
hermanntrude Posted October 30, 2009 Share Posted October 30, 2009 Or both samples have 50% of A and 50% of B and there is a 10% error in the experimental method. Link to comment Share on other sites More sharing options...
ally7890 Posted September 28, 2010 Share Posted September 28, 2010 Agreed. Retention times should remain constant when a sample is re-analysed under the same conditions (column type, column temperature, carrier gas velocity etc). So it appears that the concentrations of your sample A and B are different between Data 1 and 2. Link to comment Share on other sites More sharing options...
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