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Buffer help!

dhankl987
in Biochemistry and Molecular Biology

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I just started biochemistry and am very rusty when it comes to buffer calculations.. can anyone can offer me any tips on solving these two problems? Please? :confused:

 

1. What weight of glycine (pk1=2.4, pk2=9.6) and what volume of 0.5N HCl would you need to make 100 mL of 0.2M buffer of pH 2?

 

2. What weight of glycine and what volume of 0.5 N NaOH would you need to make 100 mL of 0.2 M buffer of pH 9.6?

 

glycine mw=75

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