ashokprabhus Posted August 14, 2007 Share Posted August 14, 2007 1.how to distinguish/separate between two light chains of immunoglobulins as they are similar in all respects-ie., they move as asingle band in SDS-PAGE how do we know on the 1st hand that 2subunits of light chain are there in the same band 2.why and how does a A+ve person have Anti-B-antibody in his sera even without prior exposure to B antigen 3.why dont we use urea in place of sds in doing PAGE? Link to comment Share on other sites More sharing options...
JackMuChabas Posted August 17, 2007 Share Posted August 17, 2007 They do not move in single bans but move in a sub page of diaslectic immune responders. Link to comment Share on other sites More sharing options...
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