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seperating vesicle and unbound protein

CM
in Biochemistry and Molecular Biology

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Hi,

 

I'm looking for the "best" method to separate lipid vesicles and unbound protein. The situation is as follows:

Some protein gets reconstituted on the vesicle, but, of course, some residual protein won't reconstitute (reconstitution in this case has an efficiency of ~80%). Now I need to remove the unbound protein (a monomer of ~35 kDa) from the solution without diluting it too much.

 

Any hints / recommendations?

 

TIA

Christian

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