Jump to content

Antibody concentration in immunized rats

Lola Vargas
in Microbiology and Immunology

Featured Replies

Hi,

 

I have a question regarding antibody concentration in immunized rats. When immunizing them with a recombinant protein, what's the "expected" concentration of IgG to my protein? I understand this must differ a lot (!), but does anyone have a feeling for what a reasonable concentration range might be? Are we talking mg/ml, ug/ml, ng/ml? What's the avarage concentration of total IgG in a rat? 3-20mg/ml?

 

Cheers,

  • 3 weeks later...

If you're using a recombinant protein, the amount of IgG formed will be relative to the immune response generated against the protein.

 

Theoretically, a benign protein wouldn't generate much of a response and you could inject tons of protein into your rat and get nothing.

 

As you stated, there is absolutely no way to tell. I suggest looking at some previously published literature.

  • 5 months later...

Yes there is a way to tell by using the gluco protein response and surmising it level of deterrence.

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.