bayoubelle Posted February 15, 2006 Share Posted February 15, 2006 I am trying to find a way to detect whether a specific kinase is activated or not. Any good kits or procedures for how to do this? Link to comment Share on other sites More sharing options...
qed Posted February 15, 2006 Share Posted February 15, 2006 I am trying to find a way to detect whether a specific kinase is activated or not. Any good kits or procedures for how to do this? if you have purified substrate protein/peptide, you can measure the activity via standard enzym kinetics. Other possibility is to use radioactiv P as a tracer and detect the phosphorylation of your substrate. Link to comment Share on other sites More sharing options...
Yggdrasil Posted February 16, 2006 Share Posted February 16, 2006 Is this an in vitro or in vivo experiment? Link to comment Share on other sites More sharing options...
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