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Kinase Activation Assays?

bayoubelle
in Biochemistry and Molecular Biology

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I am trying to find a way to detect whether a specific kinase is activated or not. Any good kits or procedures for how to do this?

I am trying to find a way to detect whether a specific kinase is activated or not. Any good kits or procedures for how to do this?

 

if you have purified substrate protein/peptide, you can measure the activity via standard enzym kinetics. Other possibility is to use radioactiv P as a tracer and detect the phosphorylation of your substrate.

Is this an in vitro or in vivo experiment?

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