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g/minute

binosh1
in Biochemistry and Molecular Biology

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This is going to sound like a stupid question but if you have 5ml of 2% starch and 1ml of 1% malt diastase and your combining with iodine to see the reaction rate, how do you work out the reaction rate as weight of starch hydrolyzed per minute?:confused:

I'm assuming you're talking about saturation kinetics.

 

You'd want to use a spectrophotometer to read the absorbance at various times. Compare to a standard curve of various starch concentrations. Plot time vs concentration on a graph and find the slope.

 

That's all I'm going to say.

 

oh yeah use ms exel or someother spreadsheet program to do it. You'd have to be insane to cruch all those numbers by hand.

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