Jump to content

Bile salts for simulated intestinal fluid?


Recommended Posts

Hi, I am trying to prepare some simulated intestinal fluid (SIF) with and without micelles. Any chance there are any research biochemists who might be familiar with this media preparation?

Most methods (and commercial products) seem to mix sodium taurocholate with lecithin to prepare SIF. However, we had purchased some "bile salts" (50/50 mix of cholate and deoxycholate) which are less expensive than the taurocholate and were wondering if this is also considered appropriate for SIF, although I only found one reference so far that uses those with lecithin.

Is there a chemical reason for using taurocholate instead of cholate/deoxycholate? Would it be considered "acceptable" to use the cholate/deoxycholate mixture, or would a reviewer expect taurocholate needs to be used?

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.