wenhu Posted March 3, 2020 Share Posted March 3, 2020 Hi, Does anyone know what plasmid could express T7 RNA polymerase in mammalian cells, I would like to transfect it with other plasmid containing Gene Of Interest under control of T7 promoter. Thanks a lot! Link to comment Share on other sites More sharing options...
Dagl1 Posted March 3, 2020 Share Posted March 3, 2020 I suppose you just transfect your cells with a T7 RNA polymerase expression plasmid (my apologies if it turns out that this doesn't work, I didn't look up any difficulties). I found: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171830/ You may want to use some of their methods. Link to comment Share on other sites More sharing options...
wenhu Posted March 4, 2020 Author Share Posted March 4, 2020 14 hours ago, Dagl1 said: I suppose you just transfect your cells with a T7 RNA polymerase expression plasmid (my apologies if it turns out that this doesn't work, I didn't look up any difficulties). I found: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171830/ You may want to use some of their methods. Thank you Dagl1, I have already noticed that. Since this is made by some lab, I wonder if there are some commercial off-the-shelf plasmid. Link to comment Share on other sites More sharing options...
Dagl1 Posted March 4, 2020 Share Posted March 4, 2020 You can always check addgene: https://www.addgene.org/59926/ (I think this one is okay but only quickly looked, please investigate before buying :)!) Thermofisher, sigma and origene also sell vectors or expression systems, however I couldn't easily find an eukaryotic expression vector there. Link to comment Share on other sites More sharing options...
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