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Capping gRNA (or other uncapped RNA)

TeslaTurbine
in Biochemistry and Molecular Biology

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Hello everyone,

I need to cap gRNA's. I've found a product online to do so. However it requires that the gRNA has a di- or triphosphate at its 5' end. I'm new to the CRISPR and RNA world in general. Do gRNA's have this di-/triphosphate at their 5' ends?

I know this seems like a relatively easy question to find out, but I've been rummaging through literature for hours trying to find this one tiny bit of information and have come up with nothing.

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