TeslaTurbine

Your gel being orange is totally normal. If you really wanted to, you could try using less EtBr, but its unlikely it will completely get rid of the orange tint. You are seeing the unintercalated EtBr. EtBr increases its fluorescence 25x when bound to dsDNA. If you have enough EtBr you will see it when its not intercalated too. You can also see that the bottom of the gel is not orange. This is because EtBr has a positive charge and migrates towards the negative electrode. If you were to publish an EtBr gel, you would use a software to filter out the background and turn it black and white anyway.

Hello everyone, I need to cap gRNA's. I've found a product online to do so. However it requires that the gRNA has a di- or triphosphate at its 5' end. I'm new to the CRISPR and RNA world in general. Do gRNA's have this di-/triphosphate at their 5' ends? I know this seems like a relatively easy question to find out, but I've been rummaging through literature for hours trying to find this one tiny bit of information and have come up with nothing.