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Is there a way to remove hemoglobin from pig kidney homogenate without deactivating enzymes?

Sigma90
in Biochemistry and Molecular Biology

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I would like to measure different enzyme activities in centrifuged pig kidney homogenate. I get out of range errors at 450 nm. The samples are still reddish after centrifugation. Is there a way to remove hemoglobin without deactivating / removing enzymes? Thanks!

Do you  want to measure the amount of each via protein quantitation, or do you want to measure the rate at which an enzyme catalyzes a particular reaction (often called its activity)?  You may need to fractionate your sample with standard protein purification techniques.

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