Jump to content

Existing user? Sign In
Sign In

Remember me Not recommended on shared computers

Forgot your password?

Or sign in with one of these services
Sign Up

Browse
- Forums
- Events
- Guidelines
- Staff
- Online Users
- Leaderboard
- More
Activity
- All Activity
- Search
- More
Leaderboard
More
- More

Home

DaKdeGO

New Members

View Profile See their activity

Posts
1
Joined
January 11, 2013
Last visited
January 16, 2013

Profile Information

Favorite Area of Science
Molecular Biology

DaKdeGO's Achievements

Lepton

Lepton (1/13)

0

Reputation

Ion Exchange: Chromatograph peak, no protein on SDS PAGE

DaKdeGO posted a topic in Biochemistry and Molecular Biology

Hello everyone, I am curious if you have had this experience before. Currently I am visiting a lab and have been getting really strange results while using their columns for protein purification. The short version is that I've been getting large absorbance peaks (500-2000mAU) on my chromatograph without any protein showing up on my SDS PAGE results. I've had this occur with two different proteins and different different buffers. Any thoughts? Thanks!
- January 11, 2013
- 3 replies

×

Browse
- Back
- Forums
- Events
- Guidelines
- Staff
- Online Users
- Leaderboard
Activity
- Back
- All Activity
- Search
Leaderboard

×

Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.