Vivalavida
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Posts posted by Vivalavida
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Hiii everyone,
I really need some help... I would like to see if my γδ T-cells are activated or not, I've heard about the γδ T-cell isolation kit from Miltenyi
BUT
the problem is that they use an anti-γδ TCR antibody for the purification, and I'm afraid that this antibody will activate the cells while fixing
thus make my results be wrong ...
What am I supposed to do ??
Do I have to analyse by excluding all the other lymphocytes and then use an activating marker or what ,?
Reallly need some help
Tks
0 -
Hiii everyone,
I really need some help... I would like to see if my γδ T-cells are activated or not, I've heard about the γδ T-cell isolation kit from Miltenyi
BUT
the problem is that they use an anti-γδ TCR antibody for the purification, and I'm afraid that this antibody will activate the cells while fixing
thus make my results be wrong ...
What am I supposed to do ??
Do I have to analyse by excluding all the other lymphocytes and then use an activating marker or what ,?
Reallly need some help
Tks
0
Transfer T lymphocyte from mice to mice
in Homework Help
Posted
Hiii again,
Can someone tell me how can I transfer some NKT lymphocyte from a mouse to another without irradiating the donor mouse...
I mean, will there be any reject because of mismatching between the donor and the recipient ? How can I prevent this ???
Tks for any help ....