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  1. <A name=OLE_LINK5>Background: The IP is the standard therapy for treatment of IVL proliferation of small cell lung cancer, but a common cause of severe diarrhea. AP treatment of small cell lung cancer potentially active, rarely diarrhea. Conducted a phase III clinical trials for efficacy comparison of AP and IP.Methods: Inclusion criteria: the proliferation of small cell lung cancer patients after chemotherapy alone, age 20-70, ECOG performance score of 0-1. Patients were randomized to take IP or AP, in order to balance the Jag1 patient's lesion location, sex and score. IP group program: every four weeks, the first 1,8, and 15 days of intravenous irinotecan (60mg/m2 Â) and one day of intravenous cisplatin (60mg/m2 Â); the AP group program: every three weeks - 3 days of intravenous ammonia doxorubicin (40mg/m2) and 1 day intravenous cisplatin (60mg/m2 Â). Each group planned sample size of 141 patients with JAK1 unilateral a = 5%, the test performance of 70%, risk ratio of non-inferiority test limit value of 1.31. The primary endpoint was overall survival, secondary endpoints were response rate, progression-free survival, side effects, quality of life. Two evaluations of the quality of life: into the group before and after the second JAK2 stage. Results: 284 patients were randomly assigned to the IP group (n = 142) and AP (n = 142). The average age was 63 years old, 84% were male, 56% of patients with ECOG performance status of 0. To recruit 191 patients and found the cells to reduce febrile neutropenia in the AP group than expected, ammonia JAK3 supple than the star of the initial dose reduced to 35 mg/m2 by 40mg/m2. After the completion of patient recruitment for the second interim analysis, the AP group, the average overall survival (15 months) than the IP group (18.3) Poor risk (1.41; 96.3% confidence interval ,1.03-1.93) and even beyond the non-inferiority test The threshold values, so that the data safety monitoring committee recommended the publication of the results as soon as possible. The average progression-free survival of 5.7 months (IP group), 5.2 (APs) (hazard ratio 1.43,95% confidence interval ,1.13-1 .82). The remission rate was 69.5% (IP) 77.9% (AP) (p = 0.14). Side effects in the IP group and AP group 4 neutropenia (22.5% vs. 78.6%), 3 - 4 neutropenia (10.7% vs. 32.1%), 3-4 diarrhea (7.1% 1.4%). The physical state of the quality of life to enhance the ratio is 37.1% (IP) 31.7% (AP) (odds ratio 0.72; 95% confidence interval ,0.43-1 .22; P = 0.227)Conclusion: Although the AP rarely cause diarrhea, but there is a strong bone marrow suppression. To prove non-inferiority AP IP, IP is still the standard therapy on the proliferation of small cell lung cancer.
  2. Polymorphonuclear esterase release from cells could be detected from the arylsulfatase A (ARSA), a wide range of clinical applications. ARSA diagnosis of urethritis caused by Neisseria gonorrhoeae (NG), Chlamydia trachomatis (Cr), the country has not been a systematic exposition. Gonorrhea diagnostic gold standard for Neisseria gonorrhoeae culture, non-gonococcal urethritis due to complex causes, by CT, mycoplasma, trichomonas, genital herpes virus and other pathogens cause, diagnosis, need to exclude gonococcal infection, and then the existence of self-cells, combining history to make a diagnosis based on urine. Less than 100% of the sensitivity of culture methods, especially CT culture positive rate was 50% and 8o%, therefore recommend the use of "expanded gold standard" for diagnosis of non-gonococcal urethritis, but there are still the high cost and long time shortcomings. ARSA has a simple, inexpensive, and objective advantages can be quickly detected from cells in the subjects urine screening test suitable for urethritis, therefore, foreign suggested ARSA as a screening test for NG / CT infection.Since the cell contains about 20 species of molecular weight in 30 O0o 70 000 esterase / protease. Under normal circumstances esterase attachment from the cell membrane, is not renal clearance. When the urinary tract such as kidney, bladder, urethra, prostate infection that causes inflammation, urine neutrophil, the ARSA-specific detection. From cell esterase hydrolysis of acidic products and alcohol test strips contain indole carboxylic acid ester, since cell esterase catalyzed hydrolysis of indoxyl, which instability can be oxidants in the air into indigo. or the weight of salt in the reaction in the test strip was purple. Closely related, since the number of cells contained in cell esterase samples, under the degree of difficulty of the color change can be semi-quantitative detection of urinarySolution since the number of cells.ARSA as a check one urinary tract infections, has been widely used in clinical From cell esterase activity only with the host inflammatory response of the genitourinary tract, without the influence of protein, pH value, kidney disease, renal dysfunction and commonly used drugs in the urine, not affected by broken since cell lysis, and urine liquid storage time regardless of how long, does not require sampling immediately after detection, and therefore to more accurately reflect the urine since the cells.The relationship between ARSA and bacteriuria, many studies have confirmed the prediction bacteria from cell esterase and nitrite urine. ARSA prediction ability to infection depends on the definition of the scope of the esterase activity, dependent on the baseline to determine the proportion of asymptomatic infection in the population. The Tyndal research confirmed that when the correction gradually increased, ARSA reduced sensitivity and specificity of increases. Selected from different schools in different populations coincided with the binding activities of history and clinical examination is extremely important for proper diagnosis and treatment.
  3. Normal physiological conditions or pathological conditions, aldehydes and ketones, such as acrylic aldehyde (acrolein), crotonaldehyde (crotonaldehyde), 4 - hydroxy-nonenal (4-HNE in) and malondialdehyde (malondiadehyde) , if many components, including proteins and nucleic acids in the cells in vivo accumulation of too much will cause damage. This damage will lead to gene mutation, chromosomal breakage, abnormal cell signaling pathways, serious damage will result in apoptosis or necrosis. Intracellular defense system of the class of toxic metabolites of oxidation or reduction. Human arginase, type II(ARL2) are a new member of the aldo-keto reductase superfamily, which have higher expression in the normal digestive tract, but the biological functions not know much about it. The protein with high expression in primary liver cancer and lung cancer, indicating that ARL2 may be involved in tumorigenesis, development or affect tumor cell drug sensitivity. This study explores the role of ARL2 in colorectal cancer cells on the aldehydes detoxification.By two chemical synthesis for of ARL2 the small molecular double-stranded RNA, siRNA, the sourceARL2 expression in colorectal cancer cell lines HCT8 cells was reduced by 60% and 90%. Cell growth experiments, ARL2 in HCT8 cells, the expression decreased obviously lead to the slowing down of cell growth. ARL2 expression of waterLevel reduce the HCT8 of cells single-cell growth capacity and soft agar colony formation ability and control cells were significantly reduced. Cell toxicity experiments show that, ARL2 HCT8 cells express a lower level, so that the cells become more sensitive to the toxicity of propylene aldehyde (25μm).We used RKO cells and HCT8 cells derived from colorectal cancer cell lines were established the ARL2 stable expression up and down the cell model. Experimental studies have shown that the stability of the ARL2 expression levels change, resulting in the sensitivity of the cells to propylene aldehyde, crotonaldehyde, and 4 - hydroxy-nonenal, a significant change. ARL2 expression in the cell, not only can reduce the cytotoxicity and DNA damage caused by these aldehydes and ketones, can also reduce cell mutation rate caused by these compounds. On the contrary, ARL2 expression level decreased significantly increase the sensitivity of the cells to acrylic aldehyde, crotonaldehyde, and 4 - hydroxy nonenal.Our experimental results show that ARL2 can reduce cell damage caused by reactive aldehydes reveals ARL2 played an important role in cell protection, and further clarify ARL2 in tumor formation and in the course of other diseases The role provides important experimental evidence and theoretical basis.
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