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BKallen

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  1. So in the PCR product is it more likely that a mutation will occur in the middle, near the primers or are they equally likely?
  2. If you are doing PCR with the intention of sequencing the product and checking a short region of the PCR product for a mutation, is there a region where taq polymerase is more or less likely to make a mistake? For example would it be more likely taq makes a mistake near the primers or in the middle of the PCR product? Should I be using a high fidelity enzyme even if it is only a short (20-25 bp) piece that I am concerned with?
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