Mrednaxe

Hello SFN I got a problem concerning an experiment conducted in chemistry. We're going to submit an paper on this experiment by Monday, help is needed if this is to be fulfilled. Alright, the experiment we conducted was to examine an enzyme-catalysed reaktion through spectroscopy. The reaction is: Pyruvate + NADH + H+ -> Lactate + NAD+ We measure the absorbancy and it's change for the solution at the wavelenght 340 nm. That done, we're going to make a few calculations, this is the problem since neither I nor anyone I've talked to in my class can help me with these calculations. The subject hasn't been covered in class. The calculations are: (Beer-Lambert law is connected somehow:) http://en.wikipedia.org/wiki/Beer-Lambert_law " M", "delta A / delta t min-1" and "v mikromol/min" " M" is something like "substrate concentration", no idea on how to get an value. "delta A / delta t min-1" is the change in absorbancy thorugh the change in time, but I don't see how we use get the unit min-1 here, help please. and the last "v mikromol/min" is the speed of the reaction, no idea on how to get an value on this. Thanks for all replies.

Thanks Mr Skeptic and John Cuthber for the replies So will the negative ion also emit light? (of some frequency) An somewhat irrelevant spoiler is that the negative ions in these salts were composed of non-metal substances. I'd like an generic answer.

Hello everyone I've recently conducted a flame test to find out some components in salts. http://en.wikipedia.org/wiki/Flame_test Our teacher informed us that the flame test only indicate to what the positive ion in the salt could be. (These are salts with an elemental substance as positive ion) The bunsen flame will change colour because some electrons in the positive ion will reach a higher orbit for a moment, and then fall back and in the process emit light of a specific energy (frequency). My question is why this will not happen to the negative ion, I've discussed this with some friends but none have been able to give an definite answer. Thanks for all replies:-)

Hello everyone I am reading a bit about Sumatomic particles and yesterday a thought struck me. What happens inside a black hole? As far as I know there's a lot of matter and antimatter in there. With all matter centered at the singularity the chances that, for an example, an proton and an antiproton collides would seem quite high, converting into energy. Take that scenario and replace it with a large number of particles. What do you think about this question that struck me? What goes on in there? Thanks for all the replies.