globaltrailblazer

Hello On a Gel electrophoresis why do the bands come out in one set of places if you use restriction enzymes seprately and then in in different places if you combine them? And in different numbers? For example EcoR I and Hind III. EcoR I has two restriction sites. Hind III has three restriction sites. Should not the two combined have five restriction sites? My gel only has three sites. And why are they in different places than the single lains in the gel? Thankyou

Hi Does anyone know why finding restriction site loci and sizes is useful? and also why finding the sequence of an intron is useful? Or any good sites that detail why. Thank you!

Hi Does anyone know why finding the sizes and locations of restriction enzyme sites is useful? And also why finding the sequence of an intron would be useful? Or else a really good, clear site with the information would be awsome. Thanks!