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About sonicscorch

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  • Birthday 11/02/1989

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  • Interests
    The heaviest guitar you will ever hear in your goddamn life.
  • Favorite Area of Science
    Biomedical Stuffs :-)
  1. Here's my outline method: • Fill 14 clean test tubes with 10ml of 10% hydrogen peroxide solution, using a small pipette and 100ml measuring cylinder. • Leave one of the test tubes at neutral pH7, and check this using a digital pH meter. If the pH does not initially register at 7, then buffer solution should be added until the solution is completely neutral. • Then, monitoring the pH with the pH meter, add either sulphuric acid or sodium hydroxide to each of the remaining test tubes, so that there is a hydrogen peroxide solution that is each pH ranging from pH1 to pH14. • Add 5ml of catalase to the first test tube, and immediately attach a bung and gas syringe to the top of the test tube. • Use a stopwatch to time the reaction for two minutes, before recording the amount of gas that has been produced and removing the gas syringe. • This should then be repeated for the remaining 13 test tubes, until results for all 14 pHs have been recorded. Cheers. Any information would be helpful.
  2. Hi guys. I'm an A Level Biology student in the UK, and I've just done an experiment to find the effect of pH on catalase. I used powdered catalase and expected the optimum pH to be 7, which I could have explained... But it came out quite clearly as 10, and I have no idea why, as it contradicts the research I have done on catalase. Can anyone explain this to me? Because I have to write it up and right now i'm completely stumped. Cheers.
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