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Everything posted by Debbie

  1. Hi, Is there any situation where one would want to use DH10B over DH5alpha competent cells? I've read that DH10B is used for methylated DNA but I'm not sure what that means.
  2. Why is inoculating directly from glycerol stocks sometimes, but not always, a bad thing to do? (Instead of streaking out the stock first then inoculating from a single colony).
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