element41skater

What i am getting is At pH 7.4: N-terminus = +1 Glu= -1 his= -1 C-terminus= -1 net charge = -2 and At pH 1.5: N-terminus = +1 Glu= +1 his= +1 C-terminus= -1 net charge = +2 Not totally sure, i matched the pH and the pK values to determine if i had a positive or negative charge

Why is the pH a concern then?

What isthe net charge of your pentapeptide at physiological pH 7.4 and pH 1.5? This is my pentapeptide: ala-leu-glu-hos-his. N-terminus has a pk~9 glu has a pk~4 his has a pk~6 c-terminus has a pk~3 How do i determine what the charges are for each amino acid, so that i can determine my net Charge? Thank you Alex