julia168

Hello, I was wondering if somebody had some experience with expression of small peptide in mammalian cells. I wanna express a transmembrane domain in mammalian cells which is about 40aa in addition to a tag peptide. I tried two vectors (pCI-neo and pcDNA3.1) to express these recombinant peptide in mammalian cells but both of them failed. I search online and found out that other groups used in vitro translation system to express transmembrane peptide. I can not find out any information about expression of small peptide in mammalian cells. Any information you provide is much appreciated. Thanks Julia