Thom

In linear regression: How can I demonstrate that expectation of hat-beta0 is beta0 ?? E(^Bo) = Bo I know that E(Yi) = Bo + B1·Xi ^Yi = ^Bo + ^B1·Xi ^B1 = SSxy / SSxx ^Bo = barY - ^B1· barX E(^B1) = B1 I would very grateful for your help.

Circular DNA is more resistant than linear DNA to DNAase action or digestion. Can you check if DNA digestion or degradation takes place in your kinetic assay? Some pieces of this DNA may act as primers.