abble

I'm hoping people will be willing to share their experiences on using yeast two hybrid libraries. There are a variety of options (e.g. commercial libraries, make your own, use libraries from another lab) and I'm wondering, as someone quite new to yeast two hybrid, what are some common pitfalls and things to avoid as well as where people have had success. If possible, please detail how the library was constructed, RNA source, complexity, etc.