zky

Dear friends, Does anyone know how to label immunoprecipitates with biotins? I remember in a paper, authors used this method to find which proteins could bind to a bait by IP (of course, you only can know the molecular weight of the proteins but sometimes it is a good enough clue). Thank you. zky

Many thanks. I have got it. The start condon is the first ATG in the inserted DNA sequence.

Hi all, I use the plasmid "pBabe" for the first time. Does anyone know where the start codon for protein expression is located? Is it in the the beginning of "truncated Gag" or it is the "ATG" in the incerted cDNA sequence? Thank you very much.

But I had tried before I posted this message. I can not find any useful information.

Hi, Does anyone know which antibiotics should be used to select the bacterial containing pCL-10A1, or pHit60, or pCG-VSVG? And what are the sequencing primers for pBabe vectors? Thank you very much.