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About DeoxyriboNucleicAcid

  • Birthday 03/15/1984

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  • Baryon

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Baryon (4/13)



  1. Thanks budullewraagh. Where would I put this afterwards?
  2. How would one go about disposing 1/2 a film canister of black powder without exploding it? I was doing an expriment, and made a lot of waste.
  3. I hear that vocabulary can be critical on the SAT, what do you guys recommend for that?
  4. Your district must have cut the budget down. That can be a b*tch. It almost happened in my HS, but luckily it did not. Unfortunately, AP/ honors classes are not required for schools to offer by most states, so they can be easily droped because of money matter and/ or low acceptance rates/ class sizes. I suppose you don't have too many electives available either. Well, good luck next year.
  5. Thanks for your replies. Other suggestions will be great too. I will not be taking the test for a year or so, but I want to prepare for the SAT early.
  6. Hello Everyone, Does anyone know of some good review books to use to prepare for the SAT? Do any of you guys have some study tips?
  7. It turned out to be weathered iron off of a ship, thanks for your comments and help
  8. Thanks for your replies inamorata and Skye, I am thinking along the lines of electroporesis, maybe even a combo of heat shock as well. I will have to get these pores as big as possible, and quickly too, to produce suction. Ill look into those companies, that will solve my first problem of getting the full genome. However, the host cell must loose it's DNA to do the experiment. QUESTION: Are there any methods of neuralising DNA inside a cell. without killing it? If the DNA is neutralized, it wont make RNA, and Rhibosomes will have nothing to do. There is a lag time between Neutralizing, and the cell haveing nothing to do, correct? Maybe the DNA will be inserted within that time frame?
  9. I have been working in the lab for a few year now, so I am fairly experienced. I believe that if I put my mind to it, I could probably pull this off (with the help of a few professors.)
  10. Hi Everyone. (I do have questions, but want to give you my background info first.) I am planning on doing an experiment to make one species of bacteria into another. I plan to: Take E. coli bacteria and extract all of their DNA; their entire genome. Then, I will take another species of bacteria (strep) and remove ALL DNA from them. I shall next take the E. coli DNA and force it into the strep. I want to see if the strep cells will indeed turn into the E. coli bacteria. None of this is finalized. The bacteria species and types may vary if it is easier to do this experiment. The DNA may be cut with enzymes, or whatever, as long as it goes into the 2nd bacteria type. Efficiency rates are not a problem, as long as there is a small or some percent of chance of success. ===== MY QUESTIONS: What are the lab methods of extracting a full "loop" of DNA from bacteria? Is there a way at all to extract all of the DNA in a cell (many cells will be used when I do this.) If the bacterial DNA is broken into many segments when extracting (if that is the only way,) will it reassemble with sticky ends inside the host (2nd) bacteria species. What are the methods of destroying/ taking out all of the DNA in a cell without killing that cell. Can it be done? What are the methods of forcing DNA into bacteria cells without it. Basically, this is like a clone, with no egg cell. ANY IDEAS/ RECOURCES WILL HELP ALOT. Has it been done before? Where? How can it be reproduced.
  11. hmmm, well I sent out an e-mail to someone at webmineral.com (pesky me), so hopefully I will get something from them.
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