newty

What happens in urodeles is a specialized occurance. It has been demonstrated that in ideal conditions, in vitro, we can cause cells to dedifferentiate into stem cell-like cells. They are not stem cells, per se, but have some multipotential capability to proliferate and differentiate into other cell types. There are literally hundreds, if not thousands, of interactions (between proteins and cells) that result in a perfect regenerate. It is not possible for every newt to perfectly regenerate every single time. Humans lack this capability to do this so naturally. Well, with the exception of children regenerating fingertips. Also, while dedifferentiation is necessary for regeneration, stem cells from neighbouring tissues contribute towards this process. It is more complicated that what the magazine is saying (but I have not read the article). There are several theories out there of why they can, but we cannot regenerate. I have commented on this in an earlier thread. Newty

"Do you know other examples of self-healing surfaces?" As said above, I am unsure as to what you are actually asking. Are you asking about complex vs simple regeneration? Epidermal wound healing is an example of simple regeneration. Limb regeneration is a complex method. Nematode regeneration, starfish regeneration are both considered to be complex regeneration, although an argument can be made for these being labeled as simple regeneration. If you can clarify what you are asking for, we can help you better. Newty

Try cutting with only one enzyme. If the culprit is a bad enzyme, then you know your double digest is not working. Maybe the double digest reaction conditions are not optimal? Try a different buffer, more enzyme, let it go longer, etc. Newty

We need more info. What kind of vector is pBabe? Was it made in a lab or bought from a company? Do you know the sequence? Is this an eukaryotic- or prokaryotic-based study? Etc etc. Most eukaryotic genes begin translation with the ATG (usually defined by a Kozak consensus sequence), but is oftentimes posttranslationally modified. There is so much info out there. More info from you would help. Also, consult a good genetics textbook as it would definetly help you. Newty

My thinking is that the LEDS are turned on when only my actinic lights are on, and that the LEDS progressively get brighter and would be at full strength by the time the actinics turn off (1.5 hours) and vice versa for the dawn effect. Would the abovementioned equipment be sufficient for that task? My only experience with these LEDS is soldering the resistors and LEDS in parallel and wiring it to a normal wall adaptor. Newty

Basically. It may be longer than that as you may have to do an industrial postdoc to be a better applicant for industrial-based jobs, and there are not too many of these postdoc positions available. It is not easy, but it is doable. The trick out of all this is to network. Talk to people, know people, talk to people, be recognized for your work, talk to people, and so on. If you are great at networking, then you may even skip the postdoc route and land a industry job after graduation. There are a lot of PhD-MBA graduates out there. If you do go through this route, you will have a leg up on them. It helps if you go to a major recognized university for your MBA as the name, unfortunately, does play a major role in the initial screening of resumes for jobs. For PhD, it is not that important if your ultimate goal is industry. Newty

I stand corrected. Newty

I apologize for hijacking this thread, but I have another question: is it possible to dim LEDS in tandem? By anology, I would like to stimulate a dusk-dawn effect. Newty

Although it is not quite antigenic, but there are plenty of bacterial strains that recognize methylated DNA and digest these DNA. Not an immunological response, just thought to be an host defense mechanism. Many of the restriction enzymes used in molecular biology work are sensitive to certain kinds of methylation. DNA itself is recognized and chewed up when cells spill out their guts (cell lysis), and there are signals that recognize this. As far as being antigenic in eukaryotes, I do not know. Newty

If we could do that, then we could stop cancer in its tracks! It is a hard task to microinject solutions into a single cell in vitro (I know because I do this for my current project), yet alone try to microinject a substance in vivo. It is too difficult, if not almost impossible. It is possible to inject into a single cell in vivo, but identification of the cell(s) is another matter altogether. Most assays are done in vitro or ex vivo (usually fixed). Newty

Maybe I didnt mean a gun-style. What i meant that the handle is like a screwdriver and the metal unit slowly tapers toward the end and the end is a very fine point. I have used it before and it worked for other small appications. I don't know the terminology, unfortunately. I only know techie words for molecular sciences! Newty

Heatsink clamp: nope. Just have a soldering gun and some wire. I guess I will have to leave at least 3 mm as mentioned below. Probably will leave around 5 mm or something like that. Thanks, Newty

A quick question here... But, first a little background: I am planning on using LEDS to make a moonlight system for my saltwater tank (I have LEDS that produce output in the correct wavelength). I am planning on putting these LEDS inside a hollow acrylic tube to waterproof the connections. The inside diameter of these tubes are short. I am wondering if I could cut off the long extending wires from the LEDS (I cannot for the life of me think of the proper term) so that it would be shorter and I could easily orientate them in the acrylic tube. Is it ok to do this? Newty

Most people in the life sciences industry recommend that you get a postgraduate degree, then try to get a job in industry (you may have to do a postdoc first before appearing as a suitable candidate, though), work for 2-3 years, then get a MBA at the best school that you can get into. Then, you are a 'hot' commodity that companies flock to. It is highly recommended to get a PhD, because there is an invisible 'glass ceiling' in industry when it comes to workers that have a BSc or MSc. The majority of people going through the ranks are those that have PhDs. The reason why they recommend working in the industry for a few years is to obtain experience and know how the system works. Then get a MBA and, you should be a highly sought after candidate. That is what most insiders say. Getting a MBA first or concurently with your PhD is not worth it, accordign to them. Newty