danyelbrooks

We've actually run into a nearly identical situation a few months back, same product line, different lot. In our case, the issues started showing up during RNA extraction, particularly when using magnetic bead protocols. The only change had been the microplate lot (also Agilent, polypropylene 1 mL, round-bottom), and we started seeing unexplained signal fluctuations and elevated background levels that hadn’t been present with previous lots. After a frustrating round of troubleshooting, we confirmed that well geometry and possible inconsistencies in irradiation or surface treatment were likely contributing factors. It might be worth checking with Agilent for a lot-specific QC report if you haven’t already. Meanwhile, as a reference point for layout consistency and cross-checking experimental setups, we found this 96 well plate template to be quite useful, especially when trying to rule out plate orientation or setup errors during high-throughput runs. Curious to know if anyone else has heard back from Agilent or had similar QC discrepancies tied to specific lots. Would be great to get more data points on this.