Tim S

I actually have read it very carefully. Thank you! By saying "very comprehensive" I meant I have never seen this long and meticulous article through I have read a lot of Promega protocols on this topic. Answering to your question- no, I have not contacted to Promega. I have read the text. And I mean their protocol specifics like "Persent Recovery" do not match to my experience of working with their Kits

Thank you for the answer! Looks very comprehensive. But the point is that, it doesn't work as the describe it unfortunately

Hey guys! 👋 Does anybody know any tip to increase DNA yield from agarose gel? I use Promega Kit and short fragments clean up properly but when it comes to linear plasmid DNA (>5000 bp), yields are poor.