Vianeye Han

I used MHA media as a medium for testing the inhibitory power of the extract against bacteria, but the research that became my reference used Nutrient agar media for the test media. and the results of our study are very different, so whether the media used can affect the results of the inhibition zone formed?

I tested the inhibitory power of the bacterium Pseudomonas aeruginosa, with the well diffusion method, I used nutrient agar for growth media and Mueller Hinton Agar as a inhibitory test medium, for the positive control I used the antibiotic ciprofloxacin and the negative control was ethanol because I used ethanol extract from Moringa leaves. Are control media and sterility tests required as well? Thanks in advance 🙏🏻

thank you so much for ur explaning Mr. CharonY, fortunately yesterday i've checked again my plates it still a bit darkening/brownish colour on my nutrient agar media and yes there is no fungal or other bacteria on it, and on my MHA media that i used for checkin bacterial inhibiton of pseudomonas turned into greenish. it's a relief that my plates did not get any contamination. Thank you once again

I want to apologize first for asking this, But I want to know if any of you know about bacterial growth media.Nutrient agar media when used as a growth medium for Pseudomonas aeruginosa during incubation for more than 24 hours, about 2 days, when viewed again, the results were that the nutrient agar media became slightly darker (became black) And the colonies that were originally white become slightly brownish. Has it been contaminated? I need help for this. Thank you🙏🏻