Stack344
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Our lab is experiencing a major issue with PCR results. We switched to a new lot of 96-well plates and suddenly started seeing rampant false signals causing QC failures.
The specific product in question is the Agilent brand: Storage/reaction microplate, 96-well, polypropylene, 1 mL/round well, round bottoms, 32 mm height, irradiated. Part Number: 204357-100.Looking to see if any other lab out there is or has experienced a similar problem, especially specifically with this equipment.
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Defective 96-well, polypropylene 1 mL round well microplate
in Equipment
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We don't use them for the qPCR reaction itself. We use them for RNA extraction and various reagent storage purposes. The plates as a whole have never been an issue, just a particular lot.