tyler1982

Hello everyone does anybody know how corning dishes are manifactured? I know the side where cells grow is treated to have some "roughness". I'd like to know their size and how they're made (electric current or whatever) Thank you all Ty

Thank you CharonY I know glass is preferable, but the protocol of this experiment is validated on plastic and to validate another one on glass would me a way too long task Best Ty

C3H cells (murine embryo fibroblasts). Dye is Lucifer Yellow CH (or any other dye used for Gap Junctions if you know any one else...) Besides, it seems petri dishes plastic has some kind of background noise when it comes to fluorescence. I've done immunofluorescence before, but only on glass coverslips. Purpose of the experiment is to observe the status of the gap junctions while a drug treatment is being done. 2 dumb questions: 1) it can only be done on single layered cells, not con multilayered colonies, right? 2) what microinjector do you use? tnx!!

Hi all! Has any one ever worked with live cell imaging? Basically I have to microinject my cells with a fluorescent dye and see what happens. If anybody has any insight about topics like - will my cell be alive and healty after the injection? - could the dye change the behaviour of my cells? - can plastic petri be used or do I need to seed on glass? Thanks in advance to anyone so kind to help!!