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Marzyme

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  1. Hello all, so for a project I need to think of a pathway to produce (R)-beta-3-aminoisobutyric acid (BAIBA), starting from either proteinogemic amino acids or glycolysis intermediates. So far I have settled on either using pyruvate or L-valine. From L-valine it is quite well known how to get the S isomer of BAIBA , but not how to get to the R enantiomer from this. So for now my sights are set on pyruvate. I can't find too much literature where enzymes are used for this, but are there any enzymes that could help attach an ethyl amine to the carbonyl center and then let the remaining hydroxyl leave as a water? Then a kinetic resolution could be used to obtain pure R-isomer. Or maybe I am going on this in a way to round about way and you know have a way better idea. So what are your ideas on this? I could really use another perspective for some inspiration.
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