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Nick 102

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August 5, 2020
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August 18, 2020

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Cloning guide RNAs

Nick 102 posted a topic in Biochemistry and Molecular Biology

Hi Guys- have a question , I’m trying to clone guide RNAs into a vector with Bfu1 sites. I was told to make an oligo that has a protospacer sequence- bfuSite/forward- reverse bfu1 site. the protospacer is the same as the forward guide sequence without the Bfu1 site i don’t understand what the protospacer is???
- August 5, 2020

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