txxplc110

Hello.
If the hydrostatic pressure in the tank is 100hPa and the external pressure is 1013hPa, then in the mercury u-tube manometer the level of manometric liquid will be:
1013hPa - 100hPa = 913hPa
913hPa = -684.8mmHg ("-" because external pressure is higher) (?)

Am I right?

Hi! Which of these points would be a dead time? 2.3 is not marked with a number so is it 3.7?

20 minutes ago, studiot said:

Not enough information.

Did you for instance have the flushing speed wrong (too fast)  ?

These are not my results and I don't have any other informations. I'm only analyzing it.  I can't publish the exact chromatogram, but it looks like the one on the picture. I am worried about the high value.

I have a question about HPLC. My peak is very small (it’s more like a little line). It is definitely not like Gauss peak, but I have to find column efficiency. Can I calculate it using the formula Ne=16*(t’r/Wb)^2? Or what else should I do? I found the information that I can only calculate it when the peak is big.

Hello! I have a question about HPLC chromatograms. I got two chromatograms, each one with different signal/wavelength (290nm and 325nm) and I got two peaks of compounds that I wanted to analyze, but the peaks are not on the same chromatogram. My question is, can I read peak resolution of two peaks from two different chromatograms, or not? It doesn’t make any sense for me, but it's in my instruction. I can’t find any information about that. I would be most grateful if you would look into this matter.