BioMed2000

We clone the gene of interest, transfect into a cell and use epitope tagging (green fluorescent protein maybe) .

fluorescence ?

I need a little help with how to go about the essay question below on a hypothetical situation. Any help would be greatly appreciated. You are working with a previously uncharacterised gene in human cells. Discuss the approaches and confirmatory steps you would employ to clone this gene to determine where the protein is localised in the cell?