BioMed2000
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We clone the gene of interest, transfect into a cell and use epitope tagging (green fluorescent protein maybe) .
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fluorescence ?
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I need a little help with how to go about the essay question below on a hypothetical situation. Any help would be greatly appreciated. You are working with a previously uncharacterised gene in human cells. Discuss the approaches and confirmatory steps you would employ to clone this gene to determine where the protein is localised in the cell?