j7235

Thanks for your response and I figure out the elution buffer is added to the centre of column only for better elution of the DNA from the membrane for centrifuge and adding it to the side may cause some of the DNA not effectively eluted from the membrane but DNA can still be extracted as long as some elution buffer is added to the membrane for centrifuge. No big deal.

I have followed the kit instruction, however, after I added the elution buffer to both of the 2 samples (from the same stem of the plant) for DNA extraction, I find out one of the AC column contains more liquid than the other one, so I pipette the excessive liquid from one AC column (for one sample) to another one (for another sample). It seems to be not a good idea, but does it mean that the two samples cannot be used? In another DNA extraction, I added the elution buffer not to the centre of the AC column (suggested by the instruction in the kit), does it mean that I better discard these samples? I only tried DNA extraction for three times... so... Wish someone could help... Thanks!