chesspuma99

Dear Sir or Madam, I do not really understand how to design a primer for a given DNA sequence. For example I have given this DNA sequence 841 aaaccagagc cggccagcca gtgaaacagc caccgtggag gggggacggc gaaaa***atg***aa 901 atccaaccaa gagcggagca acgaatgcct gcctcccaag aaacgtgaga tccccgccac ... 3241 catcgaaggc cgatctaacg tgggcaag***ta g***agaccttgc gggcagcgga ggcccggggc 3301 ctccttttac tgtctgtatc cagattactg tactgtaggc taagtaacac agtatttaca Now I want to design Primers for a PCR to amplify the region marked by the start and stop codon. How do I do that? What do I need to concern? Can you recommand any literature where there is described step-by-step what to do in this situation? And how can I add sequences for EcoRI or BamHI. I really need your help and I would be glad if someone could explain me the basic steps... Best wishes, chesspuma99