haquee3

I am looking to find out it leaves from both Piper betel and moringa trees posses any antibacterial properties. I will be testing them against: E.coli, staphylococcus aureus, enterobacteriaceae, acinetobacter baumannii, pseudomonas aeruginosa, salmonella and clostridioides difficile. I would like to hear some opinions on how to easily and quickly test both MIC and MBC. Thank you

I believe that a luminescence assay, that was suggested, may be the most suitable. Just need to find an appropriate kit to buy. Anyways, thank you for the help guys.

I am trying to screen around 600 different compounds to see if any will have antagonist properties on the human notch1 receptor, which is permenantly activated resulting in a tumor. I will be adding the max concentrations of the different compounds to the cancer tumor cells, all done on 96 well plates. The problem is how to measure the response from the different compounds. I believe the notch receptors, when activated, can be measured by gene expression. However i can not seem to find a suitable assay that will show me which compounds has inhibited the receptor. I am willing to spend around £700 for the assay. If anyone has any suggestions they will be much appreciated, thank you.