Random Particle started following Is our Alkaline Phosphatase's Km value is high or low?
Random Particle posted a topic in ChemistryHello :) We've been testing the enzyme Alkaline Phosphatase kinnetics using different concentrations of the enzyme. based on our results, we found out that our enzyme's Km is 0.831 mM. we know that a small Km indicates high affinity to the substrate and that a large Km means low affinity. We couldn't find out if our value is high or low, then we came across an article which stated that p-Nitrophenyl phosphate's Km is 1.5 mM. Does the value from the article states the optimal Km value? Does it means that our value is low and with strong affinity to the substrate? Thanks in advance!